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Welcome to The Binding of Isaac character guide. Click on a character to see its starting pickups, starting items, and achievements.
| Isaac | 3 Red Hearts | 3.5 (*1.00) | 0 | 1 | 23.75 | 1 | 0 |
| Magdalene | 4 Red Hearts | 3.5 (*1.00) | 0 | 1 | 23.75 | 0.85 | 0 |
| Cain | 2 Red Hearts | 3.5 (*1.20) | 0 | 1 | 17.75 | 1.3 | 0 |
| Judas | 1 Red Heart | 3.5 (*1.35) | 0 | 1 | 23.75 | 1 | 0 |
| Dark Judas | 2 Black Hearts | 3.5 (*2.00) | 0 | 1 | 23.75 | 1.1 | 0 |
| ??? | 3 Soul Hearts | 3.5 (*1.05) | 0 | 1 | 23.75 | 1.1 | 0 |
| Eve | 2 Red Hearts | 3.5 (*0.75) | 0 | 1 | 23.75 | 1.23 | 0 |
| Samson | 3 Red Hearts | 3.5 (*1.00) | -0.05 | 1.31 | 18.75 | 1.1 | 0 |
| Azazel | 3 Black Hearts | 3.5 (*1.50) | +0.5, *1/3 | 1 | 17.75 | 1.25 | 0 |
| Lazarus | 3 Red Hearts | 3.5 (*1.00) | 0 | 1 | 17.75 | 1 | -1 |
| Lazarus Risen | 1 Red Heart | 3.5 (*1.20) | 0 | 1 | 23.75 | 1.25 | 0 |
| Eden | Random | 3.5 | ±0.75 | 1 ± 0.25 | 23.75 ± 5.00 | 1.0 ± 0.15 | 0 ± 1 |
| The Lost | No health | 3.5 (*1.00) | 0 | 1 | 23.75 | 1 | 0 |
| Lilith | 1 Red Heart, 2 Black Hearts | 3.5 (*1.00) | 0 | 1 | 23.75 | 1 | 0 |
| Keeper | 2 Coin Hearts | 3.5 (*1.20) | 18 | 1 | 23.75 | 0.85 | -2 |
| Apollyon | 2 Red Hearts | 3.5 (*1.00) | 0 | 1 | 23.75 | 1 | 0 |
| The Forgotten | 2 Bone Hearts | 3.5 (*1.50) | 0 | 1 | 23.75 | 1 | 0 |
| The Soul | 1 Soul Heart | 3.5 (*1.00) | 0 | 1 | 23.75 | 1.3 | 0 |
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